Minds → Water & Wastewater → What must a public water system do to meet coliform sampling requirements under 40 CFR 141.21? Answer in under 100 words.
US Federal40 CFR 141.21

What must a public water system do to meet coliform sampling requirements under 40 CFR 141.21? Answer in under 100 words.

Under 40 CFR 141.21, a public water system must collect total coliform samples at sites representative of water throughout its distribution system according to a written sample siting plan, subject to State review. Routine monitoring frequency is based on population served, and systems must collect repeat samples within 24 hours of a total coliform-positive routine sample. Systems must also conduct sanitary surveys and fecal coliform or E. coli testing on total coliform-positive samples.
Last verified: 2026-08-26 · Sources checked: 1/1 resolving · Next scheduled review: 2026-11-24

Details / How it works

Public water systems must collect total coliform samples at sites representative of water throughout the distribution system, as specified in a written sample siting plan subject to State review and revision. Routine monitoring frequency for community water systems depends on the population served, with minimum monthly samples ranging from 1 for systems serving 25 to 1,000 persons, up to 480 for systems serving 3,960,001 or more persons. Non-community water systems must monitor each calendar quarter they provide water, with specific rules for systems using ground water, surface water, or ground water under the direct influence of surface water.

Numbers & thresholds

Population Served Minimum Samples per Month
25 to 1,000 1
1,001 to 2,500 2
4,101 to 4,900 5
96,001 to 130,000 100
3,960,001 or more 480

When a routine sample is total coliform-positive, systems collecting more than one routine sample per month must collect no fewer than 3 repeat samples within 24 hours. Systems collecting one routine sample per month or fewer must collect no fewer than 4 repeat samples.

Exceptions & edge cases

A system serving 25 to 1,000 persons with no history of total coliform contamination and a satisfactory sanitary survey within 5 years may have its monitoring frequency reduced by the State, but not to less than one sample per quarter. Systems using surface water or ground water under the direct influence of surface water that do not practice filtration must collect at least one sample near the first service connection each day source water turbidity exceeds 1 NTU. Special purpose samples taken for disinfection checks after pipe repairs cannot be used for compliance determination, but repeat samples taken per paragraph (b) must be used.

Sources

  1. [1]U.S. National Archives, Electronic Code of Federal Regulations — 40 CFR 141.21 — Coliform sampling · as of 2026-08-26

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